Best Central Diagnostic Lab in Bangalore | Vydehi Hospital

Central Diagnostic Lab

Clinical Laboratory Services

Health care system is no longer a simple process of examining the patient and giving him/her a prescription; over the years there has been a rapid expansion in the various branches of health care services, due to the rapid explosion of scientific medical knowledge. In this process the role of laboratory in helping the clinician make an accurate diagnosis has gained tremendous importance in bridging the gap between the patient’s presenting complaints and his treatment. Up gradation of knowledge is required to understand clinical laboratory sciences today, and clinical chemistry, molecular studies and immunohistochemistry continuous to be one of the most rapidly advancing areas of laboratory medicine, with many new tests, methodologies and measuring system being introduced with each passing year.

To ensure & build trust of all the patients and doctors the laboratory provides quality services in every section of the laboratory. All the departments are equipped with the state of art instruments and standard operating procedures have been developed and streamlined to cater to all pre-analytical, analytical and post-analytical processes and procedures. These help in ascertaining & ensuring the right procedures for patient preparation, sample collection, sample identification, transport, preservation and analysis. All the laboratory services sections at Vydehi Hospital & Research Centre are run by qualified medical professionals.

Clinical Laboratory Services

The laboratory comes under the category of a large laboratory. As it is a laboratory performing tests relating to several laboratory disciplines, such as clinical biochemistry, clinical pathology, microbiology, serology, histopathology, cytopathology ,hematology, immunology blood bank and transfusion services with a load higher than 2500 tests per day and using sophisticated instruments, such as Fully automated analyzers, chemiluminescence’s analyzers, ELIZA readers, 6 PART DIFF haematology analyzers ,Coagulation analyser , ESR analyser ,Liquid based cytology , Automated tissue processing and staining systems in Histopathology automated ,urine analyzers, Vitek and Bact D Alert , GeneXpert etc. the state of art blood bank with component facility.

Clinical Laboratory Services

The first step towards a cure is diagnosis, the faster and more accurate the diagnosis, the easier and better the cure, specially so in all cases, and invariably the diagnosis or basis for diagnosis stems from the laboratory wing of the clinical laboratory, for we here in the biochemistry wing go the extra mile by being committed to ensuring accuracy and precision in specimen handling, laboratory clinical testing and making available the latest investigation in the field, so as to facilitate faster diagnosis.

The analysis is done on unicell dxc 990 and a synchron 5 pro automated analyzer and accesses-2 the chemiluminescence’s analyser etc, using reagents, calibrators, and controls of high international standards. The laboratory staff selected to perform the tests are well experienced to understand adequately each process, the in-house laboratory quality control program, ensures continues quality results, the data generated is interpreted using scientific knowledge and professional expertise. Thus giving a helping hand to clinicians to make the accurate diagnosis.

The Pathology Wing caters to all the complete blood counts detection of anaemias and coagulopathies using the latest high end fully automated analyzers like LH-780 ,Acl Elite pro & Roller LC / MC. Even the urine chemistry and microscopy is done by fully automated analyzers like iChem Velocity & IQ200 . The PAP smears are processed using latest technology by thin prep Liquid based cytology using Nanocyt. All the specimen sections in Histopathology are processed using Leica automated tissue processing system & stained using automated autostainer from Leica. Rapid diagnosis using cryostat for aiding the surgeons in confirming or ruling out malignancy is also done.

The Microbiology Wing is equipped with 2 automated culture systems from Biomerieux – Vitek-2 & Bact D Alert to cater to the daily huge workload and early detection of pathogens, the ELISA based tests are done on ELISA reader while rapid diagnosis of Tuberculosis is done using PCR based GeneXpert system. Fungal cultures are also done on a routine basis.

Quality Assurance

The backbone of every hospital is the laboratory and the backbone of the laboratory is its effective Quality assurance (QA) programme. Quality Assurance (QA) is a comprehensive term, which refers to all aspects of operation, from preparation of the patient, to sample collection, sample analysis, recording of the result and its dispatch.
The stages of QA are:


The pre-analytical system takes care of the following aspects, quality of sample collection, labelling, transport, handling and storage. For test results are, markedly influenced by these, and each one can have a major effect on the accuracy of the result, so standard protocols are laid down, for the type of specimen and the nature of the test to be carried out.

Test ordering

Request forms are used to place the order in the hospital information system

Patient preparation, correct sampling time

Written instructions are made available to physicians, nursing staff and all other persons who send specimens to the laboratory for testing on the following points.

  • Patient preparation if sample should be a fasting one or not.
  • Time of collection e.g. for hormones, glucose.
  • Volume and type of sample
  • Type and proportion of anticoagulant required
  • Need for immediate processing
  • Special instructions for tests like lactate, ammonia, and drugs.
Specimen collection

Specimen collection blood is collected in vacationers, CSF, urine pleural fluid, pericardial fluid are collected in sterile containers.


Bar-coded labels are pasted on the tube before drawing, which has the name of the patient, age, date of birth, sex, and hospital registration number. Phlebotomy equipment and procedures all are set and followed, to facilitate drawing of sample in the right manner.

Specimen preparation

Direct centrifugation of primary tubes thus avoiding transfer into another tube and hence avoiding many errors.

Specimen storage
  • The samples are stored in closed containers, impermeable to light, or in some cases depending on the test, are aliquated and stored at appropriate temperatures, till the analysis is done. Taking care of the pre analytical storage problem.
  • As majority of the errors happen in the pre-analytical phase hence all measures are taken at every step and samples are rejected outrightly as per the rejection criteria laid down.
  • The following aspects are monitored, evaluated, implemented and maintained to ensure accuracy, precision and reliability of the tests carried out. The SOPs are made accessible to all the staff members, involved in testing and written down procedures for specific tests, are available at the workbench.
  • The sop is approved by the in charge and revised when necessary the methods used are those validated and accepted internationally and nationally.
Quality of distilled water

High quality water RO water got from the main ro plant is again made to pass through a Millipore and this water is used for analysis.

Quality of the reagents

Reagents supplied by the manufacturers are checked for linearity and stability and only after passing the internal quality checks are the reagents procured thus ensuring that sub standard reagents are not used for analysis.

Specimen collection

Specimen collection blood is collected in vacationers, CSF, urine pleural fluid, pericardial fluid are collected in sterile containers.


Soft ware programs have been created in house to get more number of tests per ml of reagent, with out compromising on the test results thus being highly cost effective.


All testing instruments, centrifuges, semi automated pipettes are calibrated at regular intervals, the calibrators used are traceable to national/ and international reference standards, precision is maintained through use of a suitable QC material commercially procured. Records of frequency of calibration, routine maintenance and daily calibration checks of instruments are present.

Quality control
  • Internal quality control and external quality assessments are being done. Quality Control material, is analyzed at predetermined intervals, level 1, 2, and 3 are used along with patient samples, to monitor systemic and random errors. The QC materials used are traceable to national /international certified reference material, so that accuracy of measurements is monitored. All data, relating to the laboratory internal quality control practices and performance, in external quality assessment schemes are recorded, reviewed and corrective action is implemented.
  • The laboratory takes part in the external quality assurance programme conducted by CMCvellore and Biorad laboratories. Written records are available to verify the results of QC and if QC out of control corrective action is taken.
Turnaround time

The throughput of the machine is 600 tests /hr. The samples are processed immediately, the reports are validated continuously and released as a result, the turn around time once it reaches the lab, is on an average 90 mts. The results of analysis are made available within this period so as to be useful for patient management.

Post Analytical

In order to avoid transcriptional errors in the results, the instrument is interfaced to the lab information system, so that the doctors are able to directly see the values in their computers.


All the tests are verified by the in charge, confidentiality of reports is maintained Results are reported in appropriate units and reference ranges are given, for each test, for the specific age and sex of the patient. These are based on primary reference as mentioned in the method.

Life threatening conditions results
  • Some urgent tests or results of life threatening conditions e.g. cardiac enzymes is communicated urgently by telephone, even though they have been released in the computer. The staff checks the name of the patient, the registration number and after communicating the report asks them to repeat the information given.
    Disposal of samples and related materials
  • The laboratory disposes contaminated waste, such as blood, body fluids, contaminated swabs, tissue papers, pipette tips, storage vials, Needles and other sharps by disinfect ion with chemicals at the workstation using sodium hypo chlorite and the rest through, authorized waste disposal agency.

The laboratory maintains the patient’s records in the system, wherein it is ensured that all the tests performed on a particular patient, can be got by the click on a single screen. To enable the doctor to compare the old reports with the latest one, to assess how the diseases is progressing. Phlebotomy equipment and procedures all are set and followed, to facilitate drawing of sample in the right manner.

Ethical practices

The laboratory maintains the highest standards of integrity, in all its operations. The laboratory maintains utmost confidentiality with regard to the findings on the specimens sent to the lab, Transparency is maintained in reporting the results to the consultants and an open invitation has been given to them to walk in any day and ask for the quality control measures taken. Any additional finding found, which will have a bearing on the patient’s treatment, or diagnosis, is reported to the physician immediately.


The Space provided is as required, wherein to commensurate with the number, type and range of tests performed the level of automation available, and degree of computerization, workload and manpower. Adequate space is also provided to enable efficient maintenance of equipment and ensuring safety of personnel.

  • Sample collection
  • Sample analysis.
  • Storage of samples, reagents, and chemicals spares, stationary and records.
  • Washing.
  • Seminar room and library
  • Staff room
  • Toilets
  • The laboratory is housed in the, as a result it is well lit and air-conditioned basement. Un-interrupted power supply is made available as the lab ups, is connected to the hospital ups

Unicell dxc 600 and a synchron 5 pro clinical chemistry system from Beckman coulter, with a through put of 990 tests/hr and 5 parameters like glucose, , electrolytes with a turn around time of 1 mit Delivers the results for 90 basic metabolic panels in an hour.

Access immunoassay system
  • From Beckman coulter is used for analysis of hormones, cardiac markers etc.
  • Beckman centrifuges 2 in these the sectors used in the analyzers can be placed directly in them and centrifuged
Pathology Section : LH-780

We at vydehi maximize our Laboratory Efficiency with 5-Part Differential Analysis. We are one of the few hematology laboratories conducting 5-part differential analysis. The COULTER AC•T™ 5diff delivers accurate test results

Acl Elite Pro
Roller LC & MC : Automated ESR Analyzers
iChem Velocity – Urine chemistry analyzer
Q200 – Urine Microscopy analyser
Thin Prep Liquid based cytology from Nanocyt
Tissue Processor, Embedding Station , Microtome , Autostainer & Cryostat from Leica
Grossing Station from Istos

Blood Bank :
The donor station

The donor station has a uniquely designed chair wherein the leg position suits the hemodynamics of the human body for ensuring safe and comfortable blood donation and minimizes the onset of vasovagal attacks.

Blood Collection Monitor

The gentle oscillation movement ensures continuous and uniform mixing of blood with anticoagulant to eliminate blood clots. This lightweight compact tube sealer conserves space in the work area and has international safety certification It produces hermetic seal by waves minimizing contamination and haemolysis of blood and blood components and the sealing head is easy to clean

1. Blood Weighing Scale
Intelligent Expressor:

The Blood/Component Weighing Scale provides accurate weight and volume of blood and its components. Intelligent Expressor: The Blood/Component Weighing Scale provides accurate weight and volume of blood and its components.

Plasma Expressor:

The optical sensor heads of the electronic plasma expressor provides precise separation with minimum contamination.

2. Blood Bank refrigerator

The unique forced airflow system ensures uniform temperature to all units of blood with maximum variation of +- 1ºC in all parts of the cabinet.

The Thermo Scientific RC12BP Low-Speed Centrifuge provides Superior separations and increased productivity reliability and safety for use in our lab and clean room environments. With the ability to run up to 12 blood bags or 12 L of sample in a single run, the Sorvall RC12BP offers high-throughput to meet the most demanding lab requirements.

40and -70 refrigerantors:

All refrigerant used are non CFC, non HCFC, non flammable and commercially easily available. Temperature range: -40°C to -70°C. With Electronic temperature control system

Plasma thawer:

The QuickThaw DH4 uses both controlled temperature and agitation to substantially reduce thaw times while ensuring the safety of your plasma.

Platelet incubator:

It is convenient and easy to operate, allowing us to load, program, and walk away. The PC100i is an i.Series countertop platelet incubator that provides the security of a continuously controlled temperature environment with an efficient use of counter space. Features Center Integrated Monitoring System with: Advanced monitoring of temperature, agitator motion, and door openings are Password protected with Automatic alarm testing at the touch of a button

Microbiology Section :
  • Bact Alert & Vitek Compact from Biomerieux
  • Laminar air flow
  • Biosafety Cabinets from Esco
  • ELISA Reader
  • GeneXpert Module IV

The laboratory runs round the clock.